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. 2004 Jul 1;32(Web Server issue):W394-9.
doi: 10.1093/nar/gkh351.

BOMP: a program to predict integral beta-barrel outer membrane proteins encoded within genomes of Gram-negative bacteria

Frode S Berven  1 , Kristian Flikka, Harald B Jensen, Ingvar Eidhammer
Affiliations

BOMP: a program to predict integral beta-barrel outer membrane proteins encoded within genomes of Gram-negative bacteria

Frode S Berven et al. Nucleic Acids Res. .

Abstract

This work describes the development of a program that predicts whether or not a polypeptide sequence from a Gram-negative bacterium is an integral beta-barrel outer membrane protein. The program, called the beta-barrel Outer Membrane protein Predictor (BOMP), is based on two separate components to recognize integral beta-barrel proteins. The first component is a C-terminal pattern typical of many integral beta-barrel proteins. The second component calculates an integral beta-barrel score of the sequence based on the extent to which the sequence contains stretches of amino acids typical of transmembrane beta-strands. The precision of the predictions was found to be 80% with a recall of 88% when tested on the proteins with SwissProt annotated subcellular localization in Escherichia coli K 12 (788 sequences) and Salmonella typhimurium (366 sequences). When tested on the predicted proteome of E.coli, BOMP found 103 of a total of 4346 polypeptide sequences to be possible integral beta-barrel proteins. Of these, 36 were found by BLAST to lack similarity (E-value score < 1e-10) to proteins with annotated subcellular localization in SwissProt. BOMP predicted the content of integral beta-barrels per predicted proteome of 10 different bacteria to range from 1.8 to 3%. BOMP is available at http://www.bioinfo.no/tools/bomp.

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Figures

Figure 1
Figure 1
Relative amino acid abundance values for the reference set. Dark spots are integral OMPs, and light spots are other proteins. The black spot inside the circle represents a protein whose five closest neighbours are inside the circle. Three are integral OMPs and two are other proteins, thus the unknown candidate is predicted to be an integral OMP. Figure created with J-Express (32).
See this image and copyright information in PMC

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