Salt-dependent studies of NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii
- PMID: 15221656
- DOI: 10.1007/s00792-004-0398-z
Salt-dependent studies of NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii
Abstract
The salt-dependent stability of recombinant dimeric isocitrate dehydrogenase [ICDH; isocitrate: NADP oxidoreductase (decarboxylating), EC 1.1.1.42] from the halophilic archaeon Haloferax volcanii (Hv) was investigated in various conditions. Hv ICDH dissociation/deactivation was measured to probe the respective effect of anions and cations on stability. Surprisingly, enzyme stability was found to be mainly sensitive to cations and very little (or not) sensitive to anions. Divalent cations induced a strong shift of the active/inactive transition towards low salt concentration. A high resistance of Hv ICDH to chemical denaturation was also found. The data were analysed and are discussed in the framework of the solvation stability model for halophilic proteins.
Similar articles
-
Complete reversal of coenzyme specificity of isocitrate dehydrogenase from Haloferax volcanii.Protein J. 2005 Jul;24(5):259-66. doi: 10.1007/s10930-005-6746-8. Protein J. 2005. PMID: 16284723
-
The effect of salts on the activity and stability of Escherichia coli and Haloferax volcanii dihydrofolate reductases.J Mol Biol. 2002 Oct 18;323(2):327-44. doi: 10.1016/s0022-2836(02)00916-6. J Mol Biol. 2002. PMID: 12381324
-
Structural features of halophilicity derived from the crystal structure of dihydrofolate reductase from the Dead Sea halophilic archaeon, Haloferax volcanii.Structure. 1998 Jan 15;6(1):75-88. doi: 10.1016/s0969-2126(98)00009-4. Structure. 1998. PMID: 9493269
-
Haloferax volcanii for biotechnology applications: challenges, current state and perspectives.Appl Microbiol Biotechnol. 2020 Feb;104(4):1371-1382. doi: 10.1007/s00253-019-10314-2. Epub 2019 Dec 20. Appl Microbiol Biotechnol. 2020. PMID: 31863144 Free PMC article. Review.
-
Control of isocitrate dehydrogenase catalytic activity by protein phosphorylation in Escherichia coli.J Mol Microbiol Biotechnol. 2005;9(3-4):132-46. doi: 10.1159/000089642. J Mol Microbiol Biotechnol. 2005. PMID: 16415587 Review.
Cited by
-
Haloarchaea: A Promising Biosource for Carotenoid Production.Adv Exp Med Biol. 2021;1261:165-174. doi: 10.1007/978-981-15-7360-6_13. Adv Exp Med Biol. 2021. PMID: 33783738
-
Biochemical and phylogenetic characterization of a monomeric isocitrate dehydrogenase from a marine methanogenic archaeon Methanococcoides methylutens.Extremophiles. 2020 Mar;24(2):319-328. doi: 10.1007/s00792-020-01156-2. Epub 2020 Jan 22. Extremophiles. 2020. PMID: 31970482
-
Characterization of alcohol dehydrogenase from the haloalkaliphilic archaeon Natronomonas pharaonis.Extremophiles. 2008 May;12(3):471-6. doi: 10.1007/s00792-007-0133-7. Epub 2008 Jan 10. Extremophiles. 2008. PMID: 18189118
-
Carotenoids from Haloarchaea and Their Potential in Biotechnology.Mar Drugs. 2015 Aug 25;13(9):5508-32. doi: 10.3390/md13095508. Mar Drugs. 2015. PMID: 26308012 Free PMC article. Review.
-
Microbial-derived salt-tolerant proteases and their applications in high-salt traditional soybean fermented foods: a review.Bioresour Bioprocess. 2023 Nov 18;10(1):82. doi: 10.1186/s40643-023-00704-w. Bioresour Bioprocess. 2023. PMID: 38647906 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
