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. 2004 Jul 7;126(26):8078-9.
doi: 10.1021/ja048113w.

Adaptability at a protein-DNA interface: re-engineering the engrailed homeodomain to recognize an unnatural nucleotide

Matthew D Simon  1 , Kevan M Shokat
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Adaptability at a protein-DNA interface: re-engineering the engrailed homeodomain to recognize an unnatural nucleotide

Matthew D Simon et al. J Am Chem Soc. .

Abstract

We explore the adaptability at a protein-DNA interface using phage display to re-engineer a homeodomain to specifically recognize a chemically synthesized, unnatural nucleotide. The engineered homeodomain preferentially binds to the modified DNA with affinity and specificity similar to those of natural homeodomains.

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