Inhibition of immunoreactive growth hormone secretion from lymphoid cell lines by dexamethasone

Abstract

The regulation of irGH secretion by the immune system was examined using lymphoid cell lines, H9 and IM9. Using a highly sensitive immunoassay, irGH secretion by H9 was negatively regulated by dexamethasone, whereas many other regulators of hGH secretion, including hormones, monoamines, and second messenger, had no measurable effect on irGH secretion. Treatment of H9 cells with dexamethasone for 48 hours could cause as high as 70% reduction in irGH secretion without affecting either cell numbers or viability. Using IM9, neither growth hormone releasing hormone nor thyrotropin releasing hormone had significant effect on either irGH steady-state level transcripts or irGH secretion. These findings suggest that irGH secretion by lymphocytes was regulated in a different manner from that by pituitary cells.