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. 2004 Jul;70(7):4384-6.
doi: 10.1128/AEM.70.7.4384-4386.2004.

Degradation and turnover of extracellular DNA in marine sediments: ecological and methodological considerations

Antonio Dell'Anno  1 , Cinzia Corinaldesi
Affiliations

Degradation and turnover of extracellular DNA in marine sediments: ecological and methodological considerations

Antonio Dell'Anno et al. Appl Environ Microbiol. 2004 Jul.

Abstract

Degradation rates of extracellular DNA determined in marine sediments were much higher than those in the water column. However, due to the high sediment DNA content, turnover times were much shorter in seawater. Results reported here provide new insights into the role of extracellular DNA in P cycling in marine ecosystems.

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Figures

FIG. 1.
FIG. 1.
Velocity (V) of poly(dɛA) degradation as a function of substrate concentration in the top 1 cm of sediments collected outside Ancona Harbor and at Goro Lagoon (a) and in surface seawater samples collected outside Ancona Harbor (b). Standard deviations are reported.
FIG. 2.
FIG. 2.
Time course analysis of poly(dɛA) degradation in sediment and seawater samples. Data, expressed in relative fluorescence units, refer to time course experiments carried out with the top 1 cm of sediments collected in Ancona Harbor and on surface seawater samples collected outside Ancona Harbor.
FIG. 3.
FIG. 3.
Time course analysis of poly(dɛA) degradation in treated (i.e., with added nucleases) and control (i.e., non-enzymatically treated) sediment samples. Data, expressed in relative fluorescence units, refer to time course experiments carried out with the top 1 cm of sediments collected outside Ancona Harbor.
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