A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

doi:10.1128/JCM.42.7.3309-3312.2004

. 2004 Jul;42(7):3309-12.

doi: 10.1128/JCM.42.7.3309-3312.2004.

A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

Patrice Francois¹, Gesuele Renzi, Didier Pittet, Manuela Bento, Daniel Lew, Stephan Harbarth, Pierre Vaudaux, Jacques Schrenzel

Affiliations

PMID: 15243102
PMCID: PMC446242
DOI: 10.1128/JCM.42.7.3309-3312.2004

A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

Patrice Francois et al. J Clin Microbiol. 2004 Jul.

. 2004 Jul;42(7):3309-12.

doi: 10.1128/JCM.42.7.3309-3312.2004.

Authors

Patrice Francois¹, Gesuele Renzi, Didier Pittet, Manuela Bento, Daniel Lew, Stephan Harbarth, Pierre Vaudaux, Jacques Schrenzel

Affiliation

¹ Genomic Research Laboratory, University of Geneva Hospitals, CH-1211 Geneva 14, Switzerland. patrice.francois@genomic.ch

PMID: 15243102
PMCID: PMC446242
DOI: 10.1128/JCM.42.7.3309-3312.2004

Abstract

We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

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References

1. Barber, M. 1961. Methicillin-resistant staphylococci. J. Clin. Pathol. 14:385-393. - PMC - PubMed
1. Chambers, H. F. 2001. The Changing epidemiology of Staphylococcus aureus? Emerg. Infect. Dis. 7:178-182. - PMC - PubMed
1. Fey, P. D., B. Said-Salim, M. E. Rupp, S. H. Hinrichs, D. J. Boxrud, C. C. Davis, B. N. Kreiswirth, and P. M. Schlievert. 2003. Comparative molecular analysis of community- or hospital-acquired methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 47:196-203. - PMC - PubMed
1. Gillet, Y., B. Issartel, P. Vanhems, J. C. Fournet, G. Lina, M. Bes, F. Vandenesch, Y. Piemont, N. Brousse, D. Floret, and J. Etienne. 2002. Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet 359:753-759. - PubMed
1. Harbarth, S., Y. Martin, P. Rohner, N. Henry, R. Auckenthaler, and D. Pittet. 2000. Effect of delayed infection control measures on a hospital outbreak of methicillin-resistant Staphylococcus aureus. J. Hosp. Infect. 46:43-49. - PubMed

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[1] Barber, M. 1961. Methicillin-resistant staphylococci. J. Clin. Pathol. 14:385-393. - PMC - PubMed

[2] Barber, M. 1961. Methicillin-resistant staphylococci. J. Clin. Pathol. 14:385-393. - PMC - PubMed

[3] Chambers, H. F. 2001. The Changing epidemiology of Staphylococcus aureus? Emerg. Infect. Dis. 7:178-182. - PMC - PubMed

[4] Chambers, H. F. 2001. The Changing epidemiology of Staphylococcus aureus? Emerg. Infect. Dis. 7:178-182. - PMC - PubMed

[5] Fey, P. D., B. Said-Salim, M. E. Rupp, S. H. Hinrichs, D. J. Boxrud, C. C. Davis, B. N. Kreiswirth, and P. M. Schlievert. 2003. Comparative molecular analysis of community- or hospital-acquired methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 47:196-203. - PMC - PubMed

[6] Fey, P. D., B. Said-Salim, M. E. Rupp, S. H. Hinrichs, D. J. Boxrud, C. C. Davis, B. N. Kreiswirth, and P. M. Schlievert. 2003. Comparative molecular analysis of community- or hospital-acquired methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 47:196-203. - PMC - PubMed

[7] Gillet, Y., B. Issartel, P. Vanhems, J. C. Fournet, G. Lina, M. Bes, F. Vandenesch, Y. Piemont, N. Brousse, D. Floret, and J. Etienne. 2002. Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet 359:753-759. - PubMed

[8] Gillet, Y., B. Issartel, P. Vanhems, J. C. Fournet, G. Lina, M. Bes, F. Vandenesch, Y. Piemont, N. Brousse, D. Floret, and J. Etienne. 2002. Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet 359:753-759. - PubMed

[9] Harbarth, S., Y. Martin, P. Rohner, N. Henry, R. Auckenthaler, and D. Pittet. 2000. Effect of delayed infection control measures on a hospital outbreak of methicillin-resistant Staphylococcus aureus. J. Hosp. Infect. 46:43-49. - PubMed

[10] Harbarth, S., Y. Martin, P. Rohner, N. Henry, R. Auckenthaler, and D. Pittet. 2000. Effect of delayed infection control measures on a hospital outbreak of methicillin-resistant Staphylococcus aureus. J. Hosp. Infect. 46:43-49. - PubMed

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A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

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A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

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