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. 2004 Jul;42(7):3309-12.
doi: 10.1128/JCM.42.7.3309-3312.2004.

A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

Patrice Francois  1 , Gesuele Renzi, Didier Pittet, Manuela Bento, Daniel Lew, Stephan Harbarth, Pierre Vaudaux, Jacques Schrenzel
Affiliations

A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements

Patrice Francois et al. J Clin Microbiol. 2004 Jul.

Abstract

We describe a novel procedure for rapid typing of the staphylococcal cassette chromosome mec element, a molecular marker allowing discrimination between community- and hospital-acquired methicillin-resistant Staphylococcus aureus (MRSA) strains. Oligonucleotides targeting the recombinase genes were type specific and used to type a collection of 399 MRSA isolates recovered during patient screening at admission. This novel assay constitutes a valuable tool for evaluating the molecular epidemiology of MRSA and adjusting infection control strategies against MRSA.

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