Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Jul;42(7):3317-20.
doi: 10.1128/JCM.42.7.3317-3320.2004.

Real-time PCR assay for detection and genotype differentiation of Giardia lamblia in stool specimens

Rebecca A Guy  1 , Chongxie Xiao, Paul A Horgen
Affiliations

Real-time PCR assay for detection and genotype differentiation of Giardia lamblia in stool specimens

Rebecca A Guy et al. J Clin Microbiol. 2004 Jul.

Abstract

Real-time PCR, using dual-labeled fluorescent probes targeting the beta-giardin gene, was used to detect Giardia lamblia in human stool specimens and to discriminate between isolates from the two major genetic assemblages of G. lamblia infective to humans, assemblages A and B.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Multiple alignments of isolates within the two primer-probe regions of the β-giardin gene: P241 (A) and P434 (B). Mismatches are shown in comparison with the sequence of the P-1 isolate. The GenBank accession numbers for the Giardia isolates are as follows: G. lamblia isolate P-1, M36728; WB, AY258617; H3, AY258616; KC8, AY072723; ISSFGF7, AY072724; Nij5, AY072725; LD18, AY072726; BAH8, AY072727; ISSFG4, AY072728; P15, AY072729; and G. muris Roberts-Thompson isolate, AY258618. Fprimer, forward primer; Rprimer, reverse primer; qPCR, real-time PCR.
See this image and copyright information in PMC

References

    1. Amar, C. F. L., P. H. Dear, S. Pedraza-Díaz, N. Looker, E. Linnane, and J. McLauchlin. 2002. Sensitive PCR-restriction fragment length polymorphism assay for detection and genotyping of Giardia duodenalis in human feces. J. Clin. Microbiol. 40:446-452. - PMC - PubMed
    1. Amar, C. F. L., P. H. Dear, and J. McLauchlin. 2003. Detection and genotyping by real-time PCR/RFLP analyses of Giardia duodenalis from faeces. J. Med. Microbiol. 52:681-683. - PubMed
    1. Ayyadevara, S., J. J. Thaden, and R. J. Shmookler Reis. 2000. Discrimination of primer 3′-nucleotide mismatch by Taq DNA polymerase during polymerase chain reaction. Anal. Biochem. 284:11-18. - PubMed
    1. Bialek, R., N. Binder, K. Dietz, A. Joachim, J. Knobloch, and U. E. Zelck. 2002. Comparison of fluorescence, antigen and PCR assays to detect Cryptosporidium parvum in fecal specimens. Diagn. Microbiol. Infect. Dis. 43:283-288. - PubMed
    1. Caccìo, S. M., M. De Giacomo, and E. Pozio. 2002. Sequence analysis of the β-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples. Int. J. Parasitol. 32:1023-1030. - PubMed

Publication types

Substances

Associated data