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. 2004 Jul;42(7):3326-8.
doi: 10.1128/JCM.42.7.3326-3328.2004.

Evaluation of a real-time PCR assay using the LightCycler system for detection of parvovirus B19 DNA

Richard S Buller  1 , Gregory Storch
Affiliations

Evaluation of a real-time PCR assay using the LightCycler system for detection of parvovirus B19 DNA

Richard S Buller et al. J Clin Microbiol. 2004 Jul.

Abstract

We evaluated the artus RealArt Parvovirus B19 LC PCR reagent (artus biotech USA, San Francisco, Calif.) for real-time PCR detection of parvovirus B19 DNA by retesting 71 specimens previously submitted to our laboratory. The artus assay, which produces a quantitative result and provides an internal PCR control, appeared to be slightly more sensitive than our conventional qualitative PCR assay.

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Figures

FIG. 1.
FIG. 1.
Fluctuations in the levels of parvovirus B19 viremia with time in three patients with persistent parvovirus infections. Day 0 represents the first day that specimens were obtained for each patient. The level of viremia is expressed in international units per milliliter of blood as determined by the artus assay. The + and − symbols in each panel represent the conventional PCR qualitative result for each specimen. Each asterisk in panel A represents the quantitative level of B19 DNA in cerebrospinal fluid specimens obtained from that patient.
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