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Comparative Study
. 2004 Aug;93(6):445-7.
doi: 10.1007/s00436-004-1157-x. Epub 2004 Jul 9.

The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens

Sumeth Termmathurapoj  1 , Saovanee Leelayoova, Pote Aimpun, Umaporn Thathaisong, Thirayost Nimmanon, Paanjit Taamasri, Mathirut Mungthin
Affiliations
Comparative Study

The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens

Sumeth Termmathurapoj et al. Parasitol Res. 2004 Aug.

Abstract

When in vitro cultivation was used as the 'gold standard' for the detection of Blastocystis hominis in stool specimens, simple smear and trichrome staining showed sensitivities of 16.7% and 40.2% and specificities of 94% and 80.4%, respectively. In vitro cultivation also enhanced PCR amplification for the detection of B. hominis in stool specimens. Our data show the usefulness of in vitro cultivation for the detection and molecular study of B. hominis in stool specimens.

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