Detection of spores of Bacillus anthracis from environment using polymerase chain reaction
Affiliations
- PMID: 15255613
Item in Clipboard
Detection of spores of Bacillus anthracis from environment using polymerase chain reaction
Indian J Exp Biol.
2003 Feb.
Abstract
A sensitive PCR based detection of Bacillus anthracis spores from environnment was standardized. Specific 1247bp amplicon could be detected with template concentration as low as 13 pg. Sensitivity was enhanced to 10 fold by nesting with second set of primers, forming 208bp amplicon. Extraction of DNA from spores purified from soil samples by aqueous polymer two-phase system followed by partial germination and freeze-thaw treatment yielded best results. Soil sample spiked with spores (8x10(2)/g of sample) could be detected with this method.
Similar articles
-
Evaluation of five commercial nucleic acid extraction kits for their ability to inactivate Bacillus anthracis spores and comparison of DNA yields from spores and spiked environmental samples.J Microbiol Methods. 2009 Jan;76(1):30-7. doi: 10.1016/j.mimet.2008.09.004. Epub 2008 Sep 13. J Microbiol Methods. 2009. PMID: 18824041
-
A simple method for the rapid removal of Bacillus anthracis spores from DNA preparations.J Microbiol Methods. 2009 Feb;76(2):212-4. doi: 10.1016/j.mimet.2008.10.009. Epub 2008 Oct 28. J Microbiol Methods. 2009. PMID: 18996156
-
Characterization of Bacillus anthracis spores isolates from soil by biochemical and multiplex PCR analysis.East Mediterr Health J. 2009 Jan-Feb;15(1):149-56. East Mediterr Health J. 2009. PMID: 19469438
-
Application of the real-time PCR for the detection of airborne microbial pathogens in reference to the anthrax spores.J Microbiol Methods. 2003 May;53(2):141-7. doi: 10.1016/s0167-7012(03)00019-8. J Microbiol Methods. 2003. PMID: 12654485 Review.
-
The monitoring and detection of Bacillus anthracis in the environment.Soc Appl Bacteriol Symp Ser. 1991;20:9S-18S. Soc Appl Bacteriol Symp Ser. 1991. PMID: 1909461 Review. No abstract available.
Cited by
-
Comprehensive Laboratory Evaluation of a Highly Specific Lateral Flow Assay for the Presumptive Identification of Bacillus anthracis Spores in Suspicious White Powders and Environmental Samples.Health Secur. 2016 Sep-Oct;14(5):351-65. doi: 10.1089/hs.2016.0041. Health Secur. 2016. PMID: 27661796 Free PMC article.
-
Diversity and antibiograms of bacterial organisms isolated from samples of household drinking-water consumed by HIV-positive individuals in rural settings, South Africa.J Health Popul Nutr. 2012 Sep;30(3):241-9. doi: 10.3329/jhpn.v30i3.12286. J Health Popul Nutr. 2012. PMID: 23082625 Free PMC article.