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. 2003 Feb;41(2):177-80.

Detection of spores of Bacillus anthracis from environment using polymerase chain reaction

Syed Imteyaz Alam  1 , Gauri Shankar Agarwal, Dev Vrat Kamboj, Ganga Prasad Rai, Lokendra Singh
Affiliations
  • PMID: 15255613

Detection of spores of Bacillus anthracis from environment using polymerase chain reaction

Syed Imteyaz Alam et al. Indian J Exp Biol. 2003 Feb.

Abstract

A sensitive PCR based detection of Bacillus anthracis spores from environnment was standardized. Specific 1247bp amplicon could be detected with template concentration as low as 13 pg. Sensitivity was enhanced to 10 fold by nesting with second set of primers, forming 208bp amplicon. Extraction of DNA from spores purified from soil samples by aqueous polymer two-phase system followed by partial germination and freeze-thaw treatment yielded best results. Soil sample spiked with spores (8x10(2)/g of sample) could be detected with this method.

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