[Effect of decidual cell conditioned media on invasion-related gene expression of ovarian tumor cell line COC1]

Abstract

Objective: To study the effect of decidual cell conditioned media (DCM) on the expression of the invasion-related gene of ovarian tumor cell line COC1.

Methods: After primary culture of the decidual cells of early and late pregnancy, DCM was extracted from the cell cultures for treatment of the ovarian tumor cell line COC1. Analysis of the invasion-related gene expression in COC1 cells was performed by way of reverse transcriptional (RT)-PCR.

Results: The COC1 expressed the mRNAs of matrix metalloproteinase 2 (MMP-2), tissue inhibitor of metalloproteinase-2 (TIMP-2) and plasminogen activator inhibitor type 1 (PAI-1), but not those of MMP-9, TIMP-1 or urokinase-type plasminogen activator (u-PA). The DCM of the early- and late-pregnancy decidual cell cultures significantly down-regulated the MMP-2 expression, and up-regulated the expression of the TIMP-2 and PAI-1 mRNA in COC1 cells in comparison with the control cells (P<0.01).

Conclusion: DCM can lower the invasive capacity of the ovarian tumor cell line COC1 by interrupting the balance between MMP-2 and TIMP-2 and between u-PA and PAI-1.