Comparative Study
doi: 10.1023/b:bile.0000025896.61490.6d.
Chitosan/gelatin composite microcarrier for hepatocyte culture
Affiliations
- PMID: 15269534
- DOI: 10.1023/b:bile.0000025896.61490.6d
Item in Clipboard
Comparative Study
Chitosan/gelatin composite microcarrier for hepatocyte culture
Biotechnol Lett.
2004 Jun.
Abstract
Solid and porous chitosan/gelatin (CG) composite microcarriers were prepared by a water-in-oil emulsion process with additional freezing and lyophilization. Adult rat hepatocytes (10(6) cells ml(-1)) attached on CG microcarriers maintained at least 15 d of viability and differentiated functions. Over 15 d, unimmobilized hepatocytes released 1.34-fold less lactate dehydrogenase (LDH), and retained 1.63-, 1.51- and 1.28-fold higher albumin secretion, urea synthesis and 7-ethoxycoumarin deethylation activities, respectively, than those on collagen-coated microcarriers. The CG matrix is therefore a promising microcarrier for hepatocyte culture.
Similar articles
-
Preparation and characterization of chitosan porous microcarriers for hepatocyte culture.Hepatobiliary Pancreat Dis Int. 2011 Oct;10(5):509-15. doi: 10.1016/s1499-3872(11)60086-6. Hepatobiliary Pancreat Dis Int. 2011. PMID: 21947725
-
Preparation of chitosan-gelatin hybrid scaffolds with well-organized microstructures for hepatic tissue engineering.Acta Biomater. 2009 Jan;5(1):453-61. doi: 10.1016/j.actbio.2008.07.002. Epub 2008 Jul 17. Acta Biomater. 2009. PMID: 18675601
-
Effectiveness of fructose-modified chitosan as a scaffold for hepatocyte attachment.Biol Pharm Bull. 1997 Dec;20(12):1290-4. doi: 10.1248/bpb.20.1290. Biol Pharm Bull. 1997. PMID: 9448106
-
Galactose-carrying polymers as extracellular matrices for liver tissue engineering.Biomaterials. 2006 Feb;27(4):576-85. doi: 10.1016/j.biomaterials.2005.06.008. Epub 2005 Aug 8. Biomaterials. 2006. PMID: 16084586 Review.
-
Minimally invasive tissue engineering composites and cell printing.IEEE Eng Med Biol Mag. 2003 Sep-Oct;22(5):84-91. doi: 10.1109/memb.2003.1256277. IEEE Eng Med Biol Mag. 2003. PMID: 14699941 Review. No abstract available.
Cited by
-
Long-term culture of primary hepatocytes: new matrices and microfluidic devices.Hepatol Int. 2014 Jan;8(1):14-22. doi: 10.1007/s12072-013-9487-3. Epub 2013 Nov 21. Hepatol Int. 2014. PMID: 26202403
-
3D Cultivation Techniques for Primary Human Hepatocytes.Microarrays (Basel). 2015 Feb 16;4(1):64-83. doi: 10.3390/microarrays4010064. Microarrays (Basel). 2015. PMID: 27600213 Free PMC article. Review.
-
Microfluidics generation of chitosan microgels containing glycerylphytate crosslinker for in situ human mesenchymal stem cells encapsulation.Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111716. doi: 10.1016/j.msec.2020.111716. Epub 2020 Nov 10. Mater Sci Eng C Mater Biol Appl. 2021. PMID: 33545868 Free PMC article.
-
Effect of PEDOT:PSS in tissue engineering composite scaffold on improvement and maintenance of endothelial cell function.J Biosci. 2018 Jun;43(2):307-319. J Biosci. 2018. PMID: 29872019
-
Biomaterials for liver tissue engineering.Hepatol Int. 2014 Apr;8(2):185-97. doi: 10.1007/s12072-013-9503-7. Epub 2013 Dec 27. Hepatol Int. 2014. PMID: 26202501
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources