Vascular control of ovarian function: ovulation, corpus luteum formation and regression

Abstract

Hemodynamic changes are involved in the cyclic remodeling of ovarian structures. A transrectal color Doppler ultrasonography was used to assess the blood flow and changes in the vasculature that take place in the follicle wall and within the corpus luteum (CL) during specific physiological events such as ovulation, CL development, and CL regression in cows. To investigate the local release of vasoactive peptides, steroid hormones, and prostaglandins (PGs) in the ovarian microenvironment, the capillary membranes (0.2mm diameter and 5-10mm length) of a microdialysis system (MDS) were implanted into the follicle wall and the CL in vitro. Furthermore, in vivo experiments were conducted with the same MDS membranes surgically implanted in follicle wall or on CL along with ovarian venous and jugular catheters to collect simultaneous, real-time information on the ovarian and systemic changes in the secretion of factors regulating vascular function. Based on the results obtained from the series of in vitro and in vivo experiments, we propose that a functional "cross-talk" occurs between the vascular components (endothelial cells) and steroidogenic cells to control follicular and luteal functions in the bovine ovary.