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. 2004 Aug;48(8):3203-6.
doi: 10.1128/AAC.48.8.3203-3206.2004.

Role of beta-lactamases and porins in resistance to ertapenem and other beta-lactams in Klebsiella pneumoniae

George A Jacoby  1 , Debra M Mills, Nancy Chow
Affiliations

Role of beta-lactamases and porins in resistance to ertapenem and other beta-lactams in Klebsiella pneumoniae

George A Jacoby et al. Antimicrob Agents Chemother. 2004 Aug.

Abstract

High-level resistance to ertapenem was produced by beta-lactamases of groups 1, 2f, and 3 in a strain of Klebsiella pneumoniae deficient in Omp35 and Omp36. From a wild-type strain producing ACT-1 beta-lactamase, ertapenem-resistant mutants for which the ertapenem MICs were up to 128 microg/ml and expression of outer membrane proteins was diminished could be selected.

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Figures

FIG. 1.
FIG. 1.
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of outer membrane proteins with protein standards of 97, 66, 49, and 30 kDa (lane A), K. pneumoniae C2 (lane B), K. pneumoniae 002(pMG251) (lane C), the K. pneumoniae 002(pMG251) ertapenem-resistant derivative for which the MIC was 4 μg/ml (lane D), and the K. pneumoniae 002(pMG251) ertapenem-resistant derivative for which the MIC was 128 μg/ml (lane E).
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