doi: 10.1385/1-59259-828-5:045.
Q-PCR in combination with ChIP assays to detect changes in chromatin acetylation
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- PMID: 15273402
- DOI: 10.1385/1-59259-828-5:045
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Q-PCR in combination with ChIP assays to detect changes in chromatin acetylation
Methods Mol Biol.
2004.
Abstract
Quantitative polymerase chain reaction (Q-PCR) allows for the accurate and reproducible determination of the amount of target DNA in a sample through the measurement of PCR product accumulation in "real time." This method determines starting target DNA quantity over a large assay dynamic range and requires no post-PCR sample manipulation. When used in combination with the method of chromatin immunoprecipitation (ChIP), the amount of protein binding to a specific region of DNA can be accurately and rapidly determined. A method for quantifying the presence of acetylated histones H3 and H4 on different regions of a target locus using Q-PCR after ChIP is described.
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