Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978 Nov 1;148(5):1440-5.
doi: 10.1084/jem.148.5.1440.

Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens

R L Evans, T J Faldetta, R E Humphreys, D M Pratt, E J Yunis, S F Schlossman

Peripheral human T cells sensitized in mixed leukocyte culture synthesize and express Ia-like antigens

R L Evans et al. J Exp Med. .

Abstract

We have studied the modulation of Ia-like antigens on the surface membrane of human T cells responding in a one-way mixed leukocyte culture. A heterologous antiserum, (anti-p23,30), which is specific to HLA-D-related antigens and which is unreactive with normal peripheral T cells or thymocytes, was found to bind significantly to all T cells transformed in mixed leukocyte culture (MLC) as determined by indirect immunofluorescence on a fluorescence-activated cell sorter 1. Furthermore, cytotoxic T cells responsible for cell-mediated lympholysis were shown to react with anti-p23,30, whereas their unactivated progenitors did not. Immunoprecipitation and sodium dodecyl sulfate polyacrylamide gel electrophoresis of a radioactive 29,000 and 34,000 dalton complex from MLC-primed T cells labeled with [35S]methionine indicated that allosensitized T cells synthesized these HLA-D-related antigens.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1288-92 - PubMed
    1. J Exp Med. 1976 Jul 1;144(1):113-22 - PubMed
    1. J Exp Med. 1976 Sep 1;144(3):699-712 - PubMed
    1. J Exp Med. 1976 Sep 1;144(3):713-25 - PubMed
    1. J Exp Med. 1975 Sep 1;142(3):694-700 - PubMed

Publication types