doi: 10.1021/ja0492539.
Doxorubicin accumulation in individually electrophoresed organelles
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- PMID: 15281791
- DOI: 10.1021/ja0492539
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Doxorubicin accumulation in individually electrophoresed organelles
J Am Chem Soc.
.
Abstract
We report the doxorubicin content in individual organelles following their capillary electrophoretic separation and illustrate that chemical accumulation at the subcellular level is highly heterogeneous. In individual mitochondria from cultured human leukemia cells DOX amount is around 50 zmol, 2 orders of magnitude higher than expected from diffusion during drug treatment, and spans 2 orders of magnitude.
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