Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Sep 15;89(18):8794-7.
doi: 10.1073/pnas.89.18.8794.

Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector

H Shizuya  1 , B Birren, U J Kim, V Mancino, T Slepak, Y Tachiiri, M Simon
Affiliations

Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector

H Shizuya et al. Proc Natl Acad Sci U S A. .

Abstract

A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of greater than 300 kilobase pairs. Individual clones of human DNA appear to be maintained with a high degree of structural stability in the host, even after 100 generations of serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and stable maintenance of inserted DNA, the BAC system may facilitate construction of DNA libraries of complex genomes with fuller representation and subsequent rapid analysis of complex genomic structure.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1992 Mar 11;20(5):1083-5 - PubMed
    1. Genomics. 1991 Nov;11(3):658-69 - PubMed
    1. DNA Cell Biol. 1991 May;10(4):301-10 - PubMed
    1. Nucleic Acids Res. 1990 Jul 11;18(13):3863-9 - PubMed
    1. Am J Hum Genet. 1990 Jan;46(1):95-106 - PubMed

Publication types

LinkOut - more resources