13C-Urea breath test, using a new compact nondispersive isotope-selective infrared spectrophotometer: comparison with mass spectrometry
- PMID: 15293132
- DOI: 10.1007/s00535-003-1357-7
13C-Urea breath test, using a new compact nondispersive isotope-selective infrared spectrophotometer: comparison with mass spectrometry
Abstract
Background: The (13)C-urea breath test ((13)C-UBT) is the most commonly used noninvasive method of detecting Helicobacter pylori infection. The isotope ratio mass spectrometer (IRMS) is the most commonly used device for this test, but the UBiT-IR300 infrared spectrophotometer, which, by comparison, is a more compact, less expensive, and easier to use analytical device, has now become widely used in the clinical setting in Japan. The objective of this study was to examine the diagnostic performance of the (13)C-UBT, using the UBiT-IR300.
Methods: A multicenter open-label study was performed, in which the (13)C-UBT was conducted using 100 mg of (13)C-urea. Analysis of (13)CO(2) in the expired breath was performed by infrared spectroscopy and mass spectrometry, and assessment of H. pylori infection was performed by culture, histological examination, and rapid urease test.
Results: In 255 cases of H. pylori infection diagnosed by biopsy methods, the (13)C-UBTs, performed with two different (13)C-ruea formulations, and using infrared spectroscopy for evaluation, showed a sensitivity of 97.7%, specificity of 98.0%, and accuracy of 97.8% (total number of evaluable cases, n = 505). The rate of agreement in the assessment of H. pylori infection between infrared spectroscopy and mass spectrometry was 100% ( n = 505). The regression equation for infrared spectroscopy to mass spectrometry was y = 0.9822x - 0.0809 ( n = 2542), with a correlation coefficient of r = 0.99989 ( P = 0.0001).
Conclusions: Diagnosis of H. pylori infection can be performed using infrared spectroscopy as well as mass spectrometry.
Similar articles
-
Comparison of isotope ratio mass spectrometry and nondispersive isotope-selective infrared spectroscopy for 13C-urea breath test.Am J Gastroenterol. 1999 May;94(5):1203-8. doi: 10.1111/j.1572-0241.1999.01067.x. Am J Gastroenterol. 1999. PMID: 10235194
-
Diagnostic accuracy of the 13C-urea breath test for childhood Helicobacter pylori infection: a multicenter Japanese study.Am J Gastroenterol. 2002 Jul;97(7):1668-73. doi: 10.1111/j.1572-0241.2002.05825.x. Am J Gastroenterol. 2002. PMID: 12135016
-
Validation of endoscopic 13C-urea breath test with nondispersive infrared spectrometric analysis in the management of Helicobacter pylori infection.Hepatogastroenterology. 2003 Mar-Apr;50(50):422-5. Hepatogastroenterology. 2003. PMID: 12749238
-
Urea breath test for Helicobacter pylori detection: present status.Trop Gastroenterol. 2004 Oct-Dec;25(4):156-61. Trop Gastroenterol. 2004. PMID: 15912972 Review.
-
The (13)C-urea breath test for non-invasive diagnosis of Helicobacter pylori infection: which procedure and which measuring equipment?Eur J Gastroenterol Hepatol. 2001 Jul;13(7):803-6. doi: 10.1097/00042737-200107000-00007. Eur J Gastroenterol Hepatol. 2001. PMID: 11474309 Review.
Cited by
-
An interoperability framework for multicentric breath metabolomic studies.iScience. 2022 Nov 15;25(12):105557. doi: 10.1016/j.isci.2022.105557. eCollection 2022 Dec 22. iScience. 2022. PMID: 36479147 Free PMC article.
-
Evaluation of a New Monoclonal Chemiluminescent Immunoassay Stool Antigen Test for the Diagnosis of Helicobacter pylori Infection: A Spanish Multicentre Study.J Clin Med. 2022 Aug 29;11(17):5077. doi: 10.3390/jcm11175077. J Clin Med. 2022. PMID: 36079007 Free PMC article.
-
Monitoring the Reaction of the Body State to Antibiotic Treatment against Helicobacter pylori via Infrared Spectroscopy: A Case Study.Molecules. 2021 Jun 7;26(11):3474. doi: 10.3390/molecules26113474. Molecules. 2021. PMID: 34200454 Free PMC article.
-
Helicobacter pylori detection and antimicrobial susceptibility testing.Clin Microbiol Rev. 2007 Apr;20(2):280-322. doi: 10.1128/CMR.00033-06. Clin Microbiol Rev. 2007. PMID: 17428887 Free PMC article. Review.
-
Evaluation of the diagnostic performance and the utility of Helicobacter pylori stool antigen lateral immunochromatography assay.Heliyon. 2022 Mar 25;8(3):e09189. doi: 10.1016/j.heliyon.2022.e09189. eCollection 2022 Mar. Heliyon. 2022. PMID: 35368526 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical