Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Mar;30(3):295-301.
doi: 10.1016/0041-0101(92)90870-b.

A bioassay for cobra cardiotoxin activity using semi-isolated cockroach heart

M J Klowden  1 , A J Vitale, M J Trumble, C R Wesson, W R Trumble
Affiliations

A bioassay for cobra cardiotoxin activity using semi-isolated cockroach heart

M J Klowden et al. Toxicon. 1992 Mar.

Abstract

A semi-isolated cockroach heart preparation was used to rapidly determine the activity of cobra cardiotoxin, monitored as a direct response on heart rate. This preparation produced a dose-response curve in the presence of active cardiotoxin and demonstrated that cardiotoxin retained its biological activity after boiling, although cardiotoxin activity was destroyed by heating in the presence of dithiothreitol. Experiments that cross-linked radiolabeled cardiotoxin to solubilized cockroach heart membranes suggested that cardiotoxin bound specifically to a 59,000 mol. wt membrane protein in this tissue.

PubMed Disclaimer

Publication types