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Review
. 2004 Aug;15(4):323-9.
doi: 10.1016/j.copbio.2004.06.001.

Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts

Stefan Becker  1 , Hans-Ulrich Schmoldt, Thorsten Michael Adams, Susanne Wilhelm, Harald Kolmar
Affiliations
Review

Ultra-high-throughput screening based on cell-surface display and fluorescence-activated cell sorting for the identification of novel biocatalysts

Stefan Becker et al. Curr Opin Biotechnol. 2004 Aug.

Abstract

Enzyme libraries displayed on the surface of microbial cells or microbeads can be screened with fluorogenic substrates that provide a physical linkage of the reaction product to the corresponding enzyme. Libraries exceeding 10(9) different variants can be quantitatively analysed and screened by flow cytometry at a rate of 30 000 cells/second. The promise of screening methods based on fluorescence-activated cell sorting for directed enzyme evolution is being realized and significantly improved enzymes have been reported recently.

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