Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Aug;42(8):3661-4.
doi: 10.1128/JCM.42.8.3661-3664.2004.

Comparison of a new lateral-flow chromatographic membrane immunoassay to viral culture for rapid detection and differentiation of influenza A and B viruses in respiratory specimens

Andreea C Cazacu  1 , Gail J Demmler, Mark A Neuman, Betty A Forbes, Sooyoung Chung, Jewel Greer, Ana E Alvarez, Robin Williams, Nadine Y Bartholoma
Affiliations

Comparison of a new lateral-flow chromatographic membrane immunoassay to viral culture for rapid detection and differentiation of influenza A and B viruses in respiratory specimens

Andreea C Cazacu et al. J Clin Microbiol. 2004 Aug.

Abstract

The performance of a new rapid lateral-flow chromatographic membrane immunoassay test kit for detection of influenza virus was evaluated and compared to that of viral culture in respiratory secretions collected from 400 adults and children seen at three large university hospitals during the recent 2003 influenza season. The rapid test provided results in 15 min, with excellent overall performance statistics (sensitivity, 94.4%; specificity, 100%; positive predictive value, 100%; negative predictive value, 97.5%). Both influenza A and B type viruses were reliably detected, with no significant difference in performance statistics noted by influenza virus type or by the center performing the test.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Test device and results obtained on the negative control and specimens positive for influenza A and B viruses.
See this image and copyright information in PMC

References

    1. Atmar, R. L., B. D. Baxter, E. A. Dominguez, and L. H. Taber. 1996. Comparison of reverse transcription-PCR with tissue culture and other rapid diagnostic assays for detection of type A influenza virus. J. Clin. Microbiol. 34:2604-2606. - PMC - PubMed
    1. Cazacu, A. C., J. Greer, M. Taherivand, and G. J. Demmler. 2003. Comparison of lateral-flow immunoassay and enzyme immunoassay with viral culture for rapid detection of influenza virus in nasal wash specimens from children. J. Clin. Microbiol. 41:2132-2134. - PMC - PubMed
    1. Cazacu, A. C., S. E. Chung, J. Greer, and G. J. Demmler. Comparison of the Directigen Flu A+B membrane enzyme immunoassay with viral culture for rapid detection of influenza A and B viruses in respiratory specimens. J. Clin. Microbiol. 42:3707-3710 - PMC - PubMed
    1. Chan, K. H., N. Maldeis, W. Pope, A. Yup, A. Ozinskas, J. Gill, W. H. Seto, K. F. Shortridge, and J. S. Peiris. 2002. Evaluation of the Directigen Flu A+B test for rapid diagnosis of influenza virus type A and B infections. J. Clin. Microbiol. 40:1675-1680. - PMC - PubMed
    1. Demmler, G. J. 2002. Laboratory diagnosis of influenza: recent advances. Semin. Pediatr. Infect. Dis. 13:85-90. - PubMed

Publication types

MeSH terms

Substances