Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2004 Aug;42(8):3831-3.
doi: 10.1128/JCM.42.8.3831-3833.2004.

In vivo acquisition of high-level resistance to imipenem in Escherichia coli

Laurent Poirel  1 , Claire Héritier, Colette Spicq, Patrice Nordmann
Affiliations
Case Reports

In vivo acquisition of high-level resistance to imipenem in Escherichia coli

Laurent Poirel et al. J Clin Microbiol. 2004 Aug.

Abstract

Four clonally related Escherichia coli strains were isolated successively from bile duct of a girl suffering from sclerosing cholangitis. One of them, selected after an imipenem-containing regimen, was resistant to carbapenems and to broad-spectrum cephalosporins due to a plasmid-mediated cephalosporinase, CMY-2, and the lack of outer membrane proteins OmpF and OmpC.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
OMP profiles of E. coli strains. OMPs were profiled by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Lanes 1 to 4 correspond to E. coli clinical isolates 1 to 4, respectively, and E. coli JM109 is a reference strain. E. coli isolates JF703 and JF701, expressing, respectively, OmpC or OmpF alone, are from reference . The molecular mass marker (M) and corresponding sizes (in kilodaltons) are indicated on the left. Horizontal arrows on the right indicate positions of the OMPs OmpC, OmpF, and OmpA.
See this image and copyright information in PMC

References

    1. Armand-Lefèvre, L., V. Leflon-Guibout, J. Bredin, F. Barguellil, A. Amor, J.-M. Pagès, and M.-H. Nicolas-Chanoine. 2003. Imipenem resistance in Salmonella enterica serovar Wien related to porin loss and CMY-4 β-lactamase production. Antimicrob. Agents Chemother. 47:1165-1168. - PMC - PubMed
    1. Avidor, B., Y. Kletter, S. Abulafia, Y. Golan, M. Ephros, and M. Giladi. 1997. Molecular diagnosis of cat scratch disease: a two-step approach. J. Clin. Microbiol. 35:1924-1930. - PMC - PubMed
    1. Bradford, P. A., C. Urban, N. Mariano, S. J. Projan, J. J. Rahal, and K. Bush. 1997. Imipenem resistance in Klebsiella pneumoniae is associated with the combination of ACT-1, a plasmid-mediated AmpC β-lactamase, and the loss of an outer membrane protein. Antimicrob. Agents Chemother. 41:563-569. - PMC - PubMed
    1. Bush, K. 2001. New β-lactamases in gram-negative bacteria: diversity and impact on the selection of antimicrobial therapy. Clin. Infect. Dis. 32:1085-1089. - PubMed
    1. Bush, K., G. A. Jacoby, and A. A. Medeiros. 1995. A functional classification scheme for β-lactamases and its correlation with molecular structure. Antimicrob. Agents Chemother. 39:1211-1233. - PMC - PubMed

Publication types