Trophoblastic cDNA cloning of porcine Pregnancy-Associated Glycoprotein genes (pPAG) and in silico analysis of coded polypeptide precursors

Abstract

Multiple Pregnancy-Associated Glycoprotein (PAG) genes, coding various chorionic products that belong to the aspartic proteinase family (AP), have been discovered in various eutherians (Placentalia). This paper presents cloning of five novel porcine PAG (pPAG) cDNAs: pPAG3, pPAG4, pPAG5, pPAG6, pPAG8 and pPAG10. All cDNAs have been identified by trophoblastic library screening on 13-17 days post coitum (dpc). These cloned and sequenced pPAG cDNAs have been deposited in GenBank database of National Institute of Heath/ National Center of Biotechnology Information (NIH/NCBI) with various accession numbers: AF315377, AF272734, AY188554, AF272735, AY373029 and one during submission. The open reading frames (ORF) of cloned cDNA sequences allowed for identification and in silico analysis of polypeptide pPAG precursors, including their identity content and various amino acid substitutions, which caused hypothetical structure changes of the pPAG precursors. In conclusion, our results concerning transcriptomal cloning and in silico analyses of the novel identified trophoblastic cDNA of the pPAG genes provided contributive data regarding porcine genome identification and physiology of the gestation in this species.