doi: 10.1107/S090744499300719X.
Structure of the crystalline complex of cytidylic acid (2'-CMP) with ribonuclease at 1.6 A resolution. Conservation of solvent sites in RNase-A high-resolution structures
Affiliations
- PMID: 15299491
- DOI: 10.1107/S090744499300719X
Item in Clipboard
Structure of the crystalline complex of cytidylic acid (2'-CMP) with ribonuclease at 1.6 A resolution. Conservation of solvent sites in RNase-A high-resolution structures
Acta Crystallogr D Biol Crystallogr.
.
Abstract
The X-ray structure of the inhibitor complex of bovine ribonuclease A with cytidylic acid (2'-CMP) has been determined at 1.6 A resolution and refined by restrained least squares to R = 0.17 for 11 945 reflections. Binding of the inhibitor molecule to the protein is confirmed to be in the productive mode associated with enzyme activity. A study of conserved solvent sites amongst high-resolution structures in the same crystal form reveals a stabilizing water cluster between the N and C termini.
Similar articles
-
Structure of the crystalline complex of deoxycytidylyl-3',5'-guanosine (3',5'-dCpdG) cocrystallized with ribonuclease at 1.9 A resolution.Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):767-71. doi: 10.1107/S0907444995001570. Acta Crystallogr D Biol Crystallogr. 1995. PMID: 15299807
-
Water-dependent domain motion and flexibility in ribonuclease A and the invariant features in its hydration shell. An X-ray study of two low-humidity crystal forms of the enzyme.Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):703-10. doi: 10.1107/S0907444994014794. Acta Crystallogr D Biol Crystallogr. 1995. PMID: 15299799
-
Multiple solvent crystal structures of ribonuclease A: an assessment of the method.Proteins. 2009 Sep;76(4):861-81. doi: 10.1002/prot.22393. Proteins. 2009. PMID: 19291738
-
X-ray refinement study on the binding of cytidylic acid (2'-CMP) to ribonuclease A.J Mol Biol. 1987 Jul 5;196(1):159-64. doi: 10.1016/0022-2836(87)90518-3. J Mol Biol. 1987. PMID: 3656443
-
X-ray structure of a ribonuclease A-uridine vanadate complex at 1.3 A resolution.Acta Crystallogr D Biol Crystallogr. 1997 May 1;53(Pt 3):290-301. doi: 10.1107/S090744499601582X. Acta Crystallogr D Biol Crystallogr. 1997. PMID: 15299932
Cited by
-
Insights to the Structural Basis for the Stereospecificity of the Escherichia coli Phytase, AppA.Int J Mol Sci. 2022 Jun 6;23(11):6346. doi: 10.3390/ijms23116346. Int J Mol Sci. 2022. PMID: 35683026 Free PMC article.
-
High-resolution crystal structures of ribonuclease A complexed with adenylic and uridylic nucleotide inhibitors. Implications for structure-based design of ribonucleolytic inhibitors.Protein Sci. 2003 Nov;12(11):2559-74. doi: 10.1110/ps.03196603. Protein Sci. 2003. PMID: 14573867 Free PMC article.
-
Ternary borate-nucleoside complex stabilization by ribonuclease A demonstrates phosphate mimicry.J Biol Inorg Chem. 2008 Feb;13(2):207-17. doi: 10.1007/s00775-007-0311-1. Epub 2007 Oct 24. J Biol Inorg Chem. 2008. PMID: 17957392 Free PMC article.
-
Solvation free energy in governing equations for DNA hybridization, protein-ligand binding, and protein folding.FEBS Open Bio. 2024 Nov;14(11):1837-1850. doi: 10.1002/2211-5463.13897. Epub 2024 Sep 17. FEBS Open Bio. 2024. PMID: 39289322 Free PMC article.
-
The structures of RNase A complexed with 3'-CMP and d(CpA): active site conformation and conserved water molecules.Protein Sci. 1994 Dec;3(12):2322-39. doi: 10.1002/pro.5560031217. Protein Sci. 1994. PMID: 7756988 Free PMC article.