Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Sep 1;10(17):2547-9.
doi: 10.3748/wjg.v10.i17.2547.

Changes of mRNA expression of enkephalin and prodynorphin in hippocampus of rats with chronic immobilization stress

Jia-Xu Chen  1 , Wei Li, Xin Zhao, Jian-Xin Yang, Hong-Yan Xu, Zhu-Feng Wang, Guang-Xin Yue
Affiliations

Changes of mRNA expression of enkephalin and prodynorphin in hippocampus of rats with chronic immobilization stress

Jia-Xu Chen et al. World J Gastroenterol. .

Abstract

Aim: To observe the changes of enkephalin mRNA and prodynorphin mRNA in hippocampus of rats induced by chronic immobilization stress.

Methods: Thirty rats were randomly divided into three groups of 10 each: the normal control group (group A), the group induced by chronic immobilization stress for 7 d (group B) and the group induced by chronic immobilization stress for 21 d (group C). The changes of the enkephalin mRNA and prodynorphin mRNA in the rat hippocampus were detected by reverse transcription-polymerase chain reaction (RT-PCR).

Results: Expression levels of enkephalin mRNA and prodynorphin mRNA in rat hippocampus were significantly increased under chronic immobilization stress, and the expression of prodynorphin mRNA in the rat hippocampus in group C was remarkably higher than that in group B (0.624 +/- 0.026; n = 5; P<0.01).

Conclusion: The increased enkephalin mRNA and prodynorphin mRNA gene expressions in rat hippocampus were involved in chronic stress.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Vaccarino AL, Kastin AJ. Endogenous opiates: 1999. Peptides. 2000;21:1975–2034. - PubMed
    1. Li W, Chen JX, Yang JX, Zhao X, Tang YT, Liu XL, Xu HY. The effect of compounds of soothing liver, invigorating spleen, tonifying kidney on the praxiology and immunological function of chronic immobilization stressed rats. Acta Laboratorium Animalis Scientia Sinica. 2003;11:33–37.
    1. Tang YT, Chen JX. Regulative effects of three TCM formulas on hypothalamus-pituitary-adrenal axis in the rats with chronic restrained stress. J Beijing Univ Traditional Chine Med. 2002;25:23–26.
    1. Harbuz MS, Lightman SL. Responses of hypothalamic and pituitary mRNA to physical and psychological stress in the rat. J Endocrinol. 1989;122:705–711. - PubMed
    1. Lightman SL, Young WS. Influence of steroids on the hypothalamic corticotropin-releasing factor and preproenkephalin mRNA responses to stress. Proc Natl Acad Sci USA. 1989;86:4306–4310. - PMC - PubMed

Publication types