Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Sep 1;10(17):2560-2.
doi: 10.3748/wjg.v10.i17.2560.

Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene

Yang Bai  1 , Ya-Li Zhang, Ye Chen, Jian-Feng Jin, Zhao-Shan Zhang, Dian-Yuan Zhou
Affiliations

Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene

Yang Bai et al. World J Gastroenterol. .

Abstract

Aim: To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA.

Methods: BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore, BabA immunogenicity was studied by animal test.

Results: DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA.

Conclusion: BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection.

PubMed Disclaimer

Figures

Figure 1
Figure 1
BabA2 DNA fragment amplified by PCR from Helicobacter pylori electrophoresed on 8 g/L agarose gel. Lane 1: Nucleotide marker; Lane 2: PCR products.
Figure 2
Figure 2
Identification of recombinant plasmid by restriction enzyme digestion. Lane 1: Nucleotide marker; Lane 2: PCR products; Lane 3: Recombinant plasmid/Not I; Lane 4: Recombinant plasmid/Not I and Nco I; Lane5: pET22b (+)/Not I.
Figure 3
Figure 3
The 100 g/L SDS-PAGE analysis of fusion protein expressed in BL21 (DE3). Lane 1: Molecular mass marker (67, 94) × 103; Lane 2: control strain BL21 (pET) before induction; Lane 3: control strain BL21 (pET) after 5-h induction with IPTG; Lane 4: BL21 (pET-BabA) cells before induction; Lane 5: BL21 (pET- BabA) cells after 3-h induction with IPTG; Lane 6: BL21 (pET- BabA) cells after 5-h induction with IPTG; Lane 7: BL21 (pET- BabA) cells periplasm protein after 3-h induction with IPTG; Lane 8: sonicated supernatant of BL21 (pET-BabA) cells after 3-h induction with IPTG; Lane 9: recombinant protein BabA purified.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Bai Y, Zhang YL, Wang JD, Lin HJ, Zhang ZS, Zhou DY. Conservative region of the genes encoding four adhesins of Helicobacter pylori: cloning, sequence analysis and biological information analysis. Di Yi Jun Yi Da Xue Xue Bao. 2002;22:869–871. - PubMed
    1. Vandenplas Y. Helicobacter pylori infection. World J Gastroenterol. 2000;6:20–31. - PMC - PubMed
    1. Bai Y, Chang SH, Wang JD, Chen Y, Zhang ZS, Zhang YL. Construction of the E.coli clone expressing adhesin BabA of Helicobacter pylori and evaluation of the adherence activity of BabA. Di Yi Jun Yi Da Xue Xue Bao. 2003;23:293–295, 309. - PubMed
    1. Morgner A, Miehlke S, Stolte M, Neubauer A, Alpen B, Thiede C, Klann H, Hierlmeier FX, Ell C, Ehninger G, et al. Development of early gastric cancer 4 and 5 years after complete remission of Helicobacter pylori associated gastric low grade marginal zone B cell lymphoma of MALT type. World J Gastroenterol. 2001;7:248–253. - PMC - PubMed
    1. Bai Y, Zhang YL, Wang JD, Zhang ZS, Zhou DY. [Construction of the non-resistant attenuated Salmonella typhimurium strain expressing Helicobacter pylori catalase] Di Yi Jun Yi Da Xue Xue Bao. 2003;23:101–105. - PubMed

Publication types