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. 2004 Sep;54(3):680-3.
doi: 10.1093/jac/dkh391. Epub 2004 Aug 12.

Enterococcus faecium N03-0072 carries a new VanD-type vancomycin resistance determinant: characterization of the VanD5 operon

David A Boyd  1 , Pamela Kibsey, Diane Roscoe, Michael R Mulvey
Affiliations

Enterococcus faecium N03-0072 carries a new VanD-type vancomycin resistance determinant: characterization of the VanD5 operon

David A Boyd et al. J Antimicrob Chemother. 2004 Sep.

Abstract

Objectives: To genotypically characterize the vancomycin resistance mechanism of Enterococcus faecium N03-0072, which was negative by PCR for the currently known van genotypes.

Methods: PCR was used to amplify the entire vancomycin resistance operon and the complete nucleotide sequence was determined by dideoxy cycle sequencing.

Results: Analysis revealed a VanD-type operon with 94% nucleotide identity to the VanD4 operon and 90% nucleotide identity to the VanD1/D3 operons. A set of universal primers was designed in order to identify all current vanD variants by PCR.

Conclusions: E. faecium N03-0072 carries a new VanD-type operon, designated VanD5.

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