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. 2004 Jun;23(3):187-91.
doi: 10.1089/1536859041224253.

Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I alpha chain

Wei-Ran Tang  1 , Nobutaka Kiyokawa, Tomoko Eguchi, Jun Matsui, Hisami Takenouchi, Daisuk Honma, Hiroshi Yasue, Shin Enosawa, Kenichi Mimori, Mitsuko Itagaki, Tomoko Taguchi, Yohko U Katagiri, Hajime Okita, Hiroshi Amemiya, Junichiro Fujimoto
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Development of novel monoclonal antibody 4G8 against swine leukocyte antigen class I alpha chain

Wei-Ran Tang et al. Hybrid Hybridomics. 2004 Jun.

Abstract

A mouse monoclonal antibody (MAb) was generated against swine leukocyte antigen (SLA) class I alpha chain. A newly developed series of MAb clones that react with pan leukocytes were selected and tested by immuno-histochemistry using SLA class I alpha chain expressing Cos-7 cells. Among them, MAb 4G8 was characterized by the following features: (1) 4G8 reacted with Cos-7 cells transfected with SLA class I alpha chain from the d haplotype, (2) 4G8 recognized epitopes that were different from those of commercially available anti-SLA class I MAbs 74-11-10 and PT85A, and (3) 4G8 could be used to immunostain frozen sections of thymus, spleen, lymph node, kidney, and liver tissues with good results.

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