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. 1992 Jan 11;20(1):97-104.
doi: 10.1093/nar/20.1.97.

A new method for the isolation of recombinant baculovirus

G Patel  1 , K Nasmyth, N Jones
Affiliations
Free PMC article

A new method for the isolation of recombinant baculovirus

G Patel et al. Nucleic Acids Res. .
Free PMC article

Abstract

An improved method for the isolation of baculovirus recombinants is described. The method involves the replication and maintenance of the baculovirus genome in the yeast Saccharomyces cerevisiae which was accomplished by the isolation of a baculovirus recombinant containing yeast ARS and CEN sequences ensuring stable replication in yeast and a URA3 selectable marker. The viral DNA maintained its ability to replicate in insect cells. An efficient and rapid selection system was set up, to isolate viral recombinants in yeast; DNA from selected yeast colonies was transfected into insect cells to obtain recombinant virus. We demonstrate the utility of this system by isolating recombinant viruses that express two different members of the CREB/ATF family of transcription factors.

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References

    1. Methods Enzymol. 1991;194:3-21 - PubMed
    1. Methods Enzymol. 1991;194:182-7 - PubMed
    1. EMBO J. 1991 Jul;10(7):1629-34 - PubMed
    1. Oncogene. 1990 Mar;5(3):295-302 - PubMed
    1. Nucleic Acids Res. 1990 May 25;18(10):2909-15 - PubMed