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. 2004 Sep 9;22(27-28):3628-41.
doi: 10.1016/j.vaccine.2004.03.028.

DNA vaccination of pigs with open reading frame 1-7 of PRRS virus

Annette Malene Barfoed  1 , Merete Blixenkrone-Møller, Merethe Holm Jensen, Anette Bøtner, Søren Kamstrup
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DNA vaccination of pigs with open reading frame 1-7 of PRRS virus

Annette Malene Barfoed et al. Vaccine. .

Abstract

We cloned all open reading frames of a Danish isolate of porcine reproductive and respiratory syndrome (PRRS) virus in DNA vaccination vectors. Pigs were vaccinated using a gene gun with each single construct (ORF1, ORF2, ORF3, ORF4, ORF5, ORF6, or ORF7) or combinations thereof. Vaccination with ORF7 consistently induced antibodies after three vaccinations, while antibodies were only sporadically detected in the remaining groups. After six vaccinations, all pigs were inoculated with PRRS virus and the post-inoculation antibody response was studied. Pigs vaccinated with ORF1 or ORF4 were primed for antibody response against NSP2 or GP4, respectively. Neutralising antibodies were detected in all pigs, with ORF5 vaccinated pigs showing the highest titres.

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