Tris buffer attenuates acetylcholine responses in Aplysia neurons
- PMID: 15317
- DOI: 10.1126/science.15317
Tris buffer attenuates acetylcholine responses in Aplysia neurons
Abstract
The commonly used buffering agent tris(hydroxymethyl)methylamine (tris) antagonizes the action of iontophoretically applied acetylcholine on neurons of Aplysia californica. Concentrations of 5 to 10 millimolar tris markedly reduced both excitatory and inhibitory responses.
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