Sequence and characterization of the met-7 gene of Neurospora crassa
- PMID: 1531800
- DOI: 10.1016/0378-1119(92)90698-o
Sequence and characterization of the met-7 gene of Neurospora crassa
Abstract
The proteins encoded by the met-7+ and met-3+ genes of Neurospora crassa are required to form a functional cystathionine-gamma-synthase (CGS). The met-7+ gene has been cloned by complementation of a met-7 mutant. The nucleotide sequence of the complementing DNA reveals the presence of a 542-amino acid open reading frame (ORF). Disruption of this ORF abolishes complementation of the met-7 mutation.
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