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. 2004 Sep 15;190(6):1127-31.
doi: 10.1086/422849. Epub 2004 Aug 17.

Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation

Suxiang Tong  1 , Jairam R Lingappa, Qi Chen, Bo Shu, Ashley C LaMonte, Byron T Cook, Charryse Birge, Shur-wern Wang Chern, Xin Liu, Renee Galloway, Le Quynh Mai, Wai Fu Ng, Jyh-Yuan Yang, Jagdish Butany, James A Comer, Stephan S Monroe, Suzanne R Beard, Thomas G Ksiazek, Dean Erdman, Paul A Rota, Mark A Pallansch, Larry J Anderson
Affiliations

Direct sequencing of SARS-coronavirus S and N genes from clinical specimens shows limited variation

Suxiang Tong et al. J Infect Dis. .

Abstract

Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients--1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.

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