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. 2004 Sep;48(9):3567-9.
doi: 10.1128/AAC.48.9.3567-3569.2004.

T2182C mutation in 23S rRNA is associated with clarithromycin resistance in Helicobacter pylori isolates obtained in Bangladesh

Rasel Khan  1 , Shamsun Nahar, Jinath Sultana, Mian Mashhud Ahmad, Motiur Rahman
Affiliations

T2182C mutation in 23S rRNA is associated with clarithromycin resistance in Helicobacter pylori isolates obtained in Bangladesh

Rasel Khan et al. Antimicrob Agents Chemother. 2004 Sep.

Abstract

Twelve clarithromycin-resistant (MIC, > or = 1 microg/ml) Helicobacter pylori isolates were analyzed for point mutations in the 23S rRNA gene. Sequence analysis of all of the resistant isolates revealed a T-to-C transition mutation at position 2182. Transformation experiments confirmed that a single T-to-C transition mutation at position 2182 is associated with clarithromycin resistance.

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Figures

FIG. 1.
FIG. 1.
Nucleotide sequence of the 1,300-bp amplicon from the 23S rRNA gene from a resistant H. pylori isolate. The arrow indicates a single point mutation, that is, a T2182C transition. The arrows at the 5′ and 3′ termini indicate primer sequences.
See this image and copyright information in PMC

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References

    1. Alarcon, T., D. Domingo, N. Prieto, and M. Lopez-Brea. 2000. Clarithromycin resistance stability in Helicobacter pylori: influence of the MIC and type of mutation in the 23S rRNA. J. Antimicrob. Chemother. 46:613-616. - PubMed
    1. Alarcon, T., D. Domingo, N. Prieto, and M. Lopez-Brea. 2000. PCR using 3′-mismatched primer to detect A2142C mutation in 23S rRNA conferring resistance to clarithromycin in Helicobacter pylori clinical isolates. J. Clin. Microbiol. 38:923-925. - PMC - PubMed
    1. Debets-Ossenkopp, Y. J., A. J. Herscheid, R. G. Pot, E. J. Kuipers, J. G. Kusters, and C. M. Vandenbroucke-Grauls. 1999. Prevalence of Helicobacter pylori resistance to metronidazole, clarithromycin, amoxycillin, tetracycline and trovafloxacin in The Netherlands. J. Antimicrob. Chemother. 43:511-515. - PubMed
    1. Debets-Ossenkopp, Y. J., M. Sparrius, J. G. Kusters, J. J. Kolkmann, and C. M. J. E. Vandenbroucke-Grauls. 1996. Mechanism of clarithromycin resistance in clinical isolates of Helicobacter pylori. FEMS Microbiol. Lett. 142:37-42. - PubMed
    1. European Helicobacter pylori Study Group. 1997. Current European concepts in the management of Helicobacter pylori infection. Gut 41:8-13. - PMC - PubMed

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