In situ hybridization of the mRNA for interferon-gamma, interferon-alpha E, interferon-beta, interleukin-1 beta and interleukin-6 and characterization of infiltrating cells in thyroid tissues
- PMID: 1532976
- DOI: 10.1016/0022-1759(92)90177-u
In situ hybridization of the mRNA for interferon-gamma, interferon-alpha E, interferon-beta, interleukin-1 beta and interleukin-6 and characterization of infiltrating cells in thyroid tissues
Abstract
Cytokine mRNA production in the thyroid tissues of patients with various thyroid diseases was analysed by in situ hybridization. In addition, infiltrating leukocytes were characterized by immunohistologic studies using the alkaline phosphatase anti-alkaline phosphatase (APAAP) staining technique. The following clinical material was investigated: two cases of Graves' disease, one with high and the other with a low amount of infiltrating leukocytes as well as two cases of non-toxic goitre also showing considerable quantities of infiltrating cells. The hybridization was performed on tissue sections with antisense probes for interferon-gamma (IFN-gamma), IFN-alpha E, IFN-beta, interleukin-6 (IL-6) and IL-1 beta. A small number of individual cells were found to express high levels of mRNA for IFN-gamma, IL-1 beta and measurable amounts of IL-6 throughout the tissue sections. However, IFN-alpha E or IFN-beta were not detected. Cytokine expressing cells were noted in the tissue of one patient with Graves' disease and in two cases with non-toxic goitre. In these samples a high amount of infiltrating leukocytes (CD45+) was detected, especially CD3+, CD8+, CD4+ and CD45RA+ T cells, in addition to B cells and macrophages. In one case an unusually large amount of T cell receptor gamma/delta+ (TcR gamma/delta+) cells was found. However, one sample of thyroid tissue derived from a patient with Graves' disease was poorly infiltrated and showed few cells expressing cytokines. In conclusion, using thyroid tissue as an example, our data suggest that the application of in situ hybridization with antisense RNA permits the study of cytokine production in tissues of both autoimmune and non-autoimmune origin.
Similar articles
-
Analysis of cytokine gene expression in Graves' disease and multinodular goiter.J Clin Endocrinol Metab. 1994 Aug;79(2):355-60. doi: 10.1210/jcem.79.2.8045947. J Clin Endocrinol Metab. 1994. PMID: 8045947
-
Detection of interleukin-6 and interleukin-1 production in human thyroid epithelial cells by non-radioactive in situ hybridization and immunohistochemical methods.Clin Exp Immunol. 1991 Feb;83(2):314-9. doi: 10.1111/j.1365-2249.1991.tb05634.x. Clin Exp Immunol. 1991. PMID: 1993363 Free PMC article.
-
Intrathyroidal cytokine gene expression profiles in autoimmune thyroiditis.J Endocrinol. 1994 May;141(2):309-15. doi: 10.1677/joe.0.1410309. J Endocrinol. 1994. PMID: 8046301
-
Chemokine (C-X-C motif) ligand (CXCL)10 in autoimmune diseases.Autoimmun Rev. 2014 Mar;13(3):272-80. doi: 10.1016/j.autrev.2013.10.010. Epub 2013 Nov 2. Autoimmun Rev. 2014. PMID: 24189283 Review.
-
Cytokines and Graves' disease.Baillieres Clin Endocrinol Metab. 1997 Oct;11(3):481-97. doi: 10.1016/s0950-351x(97)80708-2. Baillieres Clin Endocrinol Metab. 1997. PMID: 9532335 Review.
Cited by
-
The gamma delta T cell repertoire in Graves' disease and multinodular goitre.Clin Exp Immunol. 1993 Dec;94(3):473-7. doi: 10.1111/j.1365-2249.1993.tb08220.x. Clin Exp Immunol. 1993. PMID: 8252809 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous