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. 1978 Oct 31;44(2):173-80.
doi: 10.1007/BF00295410.

Rapid identification of chromosomes carrying silver-stained nucleolus-organizing regions: application to case of 21/21 Robertsonian translocation

C H Buys, J Osinga, W L Gouw, G J Anders

Rapid identification of chromosomes carrying silver-stained nucleolus-organizing regions: application to case of 21/21 Robertsonian translocation

C H Buys et al. Hum Genet. .

Abstract

The use of a combination of transmitted light and epiluminescence after silver and fluorescent staining of chromosome preparations makes it possible to achieve simultaneous visualization of silver-stained NORs and flourescent chromosomes. This technique permits exact localization of silver precipitates on normal and BrdU-substituted chromosomes. After previous silver impregnation, fluorescent staining by actinomycin-daunomycin-DAPI was used to induce a banding pattern that enables identification of specific chromosomes while observing silver-stained NORs at the same time. Application of this method to a Down's syndrome patient revealed a 21/21 Robertsonian translocation with NOR'S eliminated.

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References

    1. Experientia. 1975 Feb 15;31(2):260-2 - PubMed
    1. Chromosoma. 1977 Mar 7;60(1):15-25 - PubMed
    1. Chromosoma. 1977 May 23;61(3):207-14 - PubMed
    1. Hum Genet. 1976 Oct 28;34(2):199-206 - PubMed
    1. Hum Genet. 1977 Apr 15;36(2):167-72 - PubMed