Lansoprazole ameliorates intestinal mucosal damage induced by ischemia-reperfusion in rats

Abstract

Aim: To investigate the protective effect of lansoprazole on ischemia and reperfusion (I/R)-induced rat intestinal mucosal injury in vivo.

Methods: Intestinal damage was induced by clamping both the superior mesenteric artery and the celiac trunk for 30 min followed by reperfusion in male Sprague-Dawley rats. Lansoprazole was given to rats intraperitoneally 1 h before vascular clamping.

Results: Both the intraluminal hemoglobin and protein levels, as indices of mucosal damage, significantly increased in I/R-groups comparison with those of sham-operation groups. These increases in intraluminal hemoglobin and protein levels were significantly inhibited by the treatment with lansoprazole at a dose of 1 mg/kg. Small intestine exposed to I/R resulted in mucosal inflammation that was characterized by significant increases in thiobarbituric acid-reactive substances (TBARS), tissue-associated myeloperoxidase activity (MPO), and mucosal content of rat cytokine-induced neutrophil chemoattractant-1 (CINC-1). These increases in TBARS, MPO activities and CINC-1 content in the intestinal mucosa after I/R were all inhibited by pretreatment with lansoprazole at a dose of 1 mg/kg. Furthermore, the CINC-1 mRNA expression was increased during intestinal I/R, and this increase in mRNA expression was inhibited by treatment with lansoprazole.

Conclusion: Lansoprazole inhibits lipid peroxidation and reduces development of intestinal mucosal inflammation induced by I/R in rats, suggesting that lansoprazole may have a therapeutic potential for I/R injury.

Figure 1

Effect of lansoprazole on the level of thiobarbituric-acid reactive substances (TBARS), mucosal mye-loperoxidase (MPO) activity, and cytolline induced meutrophil chemo-attracttant-1 (CINC-1) in intestinal mucosa of I/R-induced rats. A: Effect of lansoprazole on the level of TBARS in intestinal mucosa of I/R-induced rats. ^aP < 0.05 vs normal; ^cP < 0.05 vs control. Sham group and I/R group received an equivalent volume of vehicle instead of lansoprazole. B: Effect of lansoprazole on the level of mucosal myeloperoxidase (MPO) activities in intestinal mucosa of I/R-induced rats. ^aP < 0.05 vs normal; ^cP < 0.05 vs control. Sham group and I/R group received an equivalent volume of vehicle instead of lansoprazole. C: Effect of lansoprazole on cytokine induced neutrophil chemoattractant-1 (CINC-1) of intestinal mucosa of I/R-induced rats. ^aP < 0.05 vs normal; ^cP < 0.05 vs control. LAN(+): with lansoprazole; LAN(-): without lansoprazole.

Figure 2

Intestinal CINC-1 mRNA expression during I/R in rats. M: Marker; Lane 1: Before ischemia; Lane 2: After I/R; Lane 3: I/R + lansoprazole.