Expression of con genes along the three sporulation pathways of Neurospora crassa

Abstract

The filamentous fungus Neurospora crassa produces three types of spores by using different developmental pathways: macroconidiation, microconidiation, and sexual spore (ascospore) formation. Several genes of unknown function have been cloned by virtue of their expression during macroconidiation but not during mycelial growth (con genes). It had been postulated that expression of the con genes was specific to macroconidiation. To test this assumption, protein extracts from macroconidia, microconidia, ascospores, and protoperithecia (sexual structures) were analyzed for the product of one of the con genes, con-10, by immunoblotting using a CON10-specific antiserum. CON10 was detected in all of these extracts. An immunologically related protein was detected in an extract from ascospores of a nonconidiating Neurospora species, N. africana. Total RNA isolated from the three types of N. crassa spores was analyzed for con gene mRNA by Northern blotting using five different con genes as probes. Transcripts for four of the genes were detected in all three spore types; mRNA for the fifth gene was detected in macroconidia and microconidia but not in ascospores. Analysis of aconidial and female sterile mutants showed that expression of the con genes along any one developmental pathway occurs when expression along another pathway is genetically blocked.