Blomia tropicalis and Dermatophagoides pteronyssinus mites evoke distinct patterns of airway cellular influx in type I hypersensitivity murine model

Abstract

Murine models of hypersensitivity to allergens are useful tools for the evaluation of strategies to downmodulate IgE response. We sought to compare allergen inflammatory pulmonary response in previously sensitized mice orally administered with dust mites Blomia tropicalis (Bt) or Dermatophagoides pteronyssinus (Dp). Sensitized A/Sn mice fed with Bt or Dp showed a significant decrease in the IgE response compared to control-immunized mice. Bt-immunized mice demonstrated an accumulation of neutrophils in the bronchoalveolar lavage fluid, while Dp-immunized mice revealed an intense influx of eosinophils in the airway. Bt oral administration did not attenuate cell influx in the airway and Dp-fed mice showed a significant decrease of neutrophils and lymphocytes in the bronchoalveolar lavage fluid. These findings demonstrated that oral tolerance induction to Bt and Dp extract in sensitized mice decrease IgE response, but does not interfere in local inflammatory pulmonary response. The distinct profile of airway cellular infiltration between mites immunization suggest an interesting model to study allergic inflammation.