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Comment
. 2004 Sep;42(9):4414; author reply 4414-5.
doi: 10.1128/JCM.42.9.4414-4415.2004.

Considerations in evaluating the applicability of universal detection of oral pathogens

Khalil Boutaga, Arie Jan van Winkelhoff, Christina M J E Vandenbroucke-Grauls, Paul H M Savelkoul
Comment

Considerations in evaluating the applicability of universal detection of oral pathogens

Khalil Boutaga et al. J Clin Microbiol. 2004 Sep.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Alignment of the 16S rRNA sequence region described by Yoshida et al., including six oral bacteria compared to the E. coli sequence. The locations of the universal primers (Uni152-F and Uni220-R) and the probe (Uni177-T) are identical to the E. coli sequence as indicated. Strains: 1, DH5α; 2, W83; 3, ATCC 29523; 4, ATCC 43037; 5, ATCC 33270, 6, ATCC 25611; 7, ATCC 10953. Dots, nucleotides identical to those in the E. coli sequence; dashes, deletions.
FIG. 1.
FIG. 1.
Alignment of the 16S rRNA sequence region described by Yoshida et al., including six oral bacteria compared to the E. coli sequence. The locations of the universal primers (Uni152-F and Uni220-R) and the probe (Uni177-T) are identical to the E. coli sequence as indicated. Strains: 1, DH5α; 2, W83; 3, ATCC 29523; 4, ATCC 43037; 5, ATCC 33270, 6, ATCC 25611; 7, ATCC 10953. Dots, nucleotides identical to those in the E. coli sequence; dashes, deletions.
See this image and copyright information in PMC

Comment on

References

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    1. Greisen, K., M. Loeffelholz, A. Purohit, and D. Leong. 1994. PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid. J. Clin. Microbiol. 32:335-351. - PMC - PubMed
    1. Huijsdens, X. W., R. K. Linskens, M. Mak, S. G. Meuwissen, C. M. Vandenbroucke-Grauls, and P. H. Savelkoul. 2002. Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR. J. Clin. Microbiol. 40:4423-4427. - PMC - PubMed
    1. Yoshida, A., N. Suzuki, Y. Nakano, T. Oho, M. Kawada, and T. Koga. 2003. Development of a 5′ fluorogenic nuclease-based real-time PCR assay for quantitative detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. J. Clin. Microbiol. 41:863-866. - PMC - PubMed
    1. Corless, C. E., M. Guiver, R. Borrow, V. Edwards-Jones, E. B. Kaczmarski, and A. J. Fox. 2000. Contamination of and sensitivity issues with a real-time universal 16S rRNA PCR. J. Clin. Microbiol. 38:1747-1752. - PMC - PubMed

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