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. 2004 Oct;216(Pt 1):49-51.
doi: 10.1111/j.0022-2720.2004.01383.x.

A common aberration with water-immersion objective lenses

R Arimoto  1 , J M Murray
Affiliations

A common aberration with water-immersion objective lenses

R Arimoto et al. J Microsc. 2004 Oct.

Abstract

We describe here an aberration that is frequently encountered with water-immersion but not oil-immersion objectives. The aberration is shown to be induced by tilt of the coverslip out of the plane normal to the optical axis. Model calculations taking into account the path-length distortions introduced by a tilted coverslip satisfactorily reproduce the observed effect on the images of small subresolution fluorescent beads.

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