doi: 10.1084/jem.91.1.1.
Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody
- PMID: 15395569
- PMCID: PMC2135948
- DOI: 10.1084/jem.91.1.1
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Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody
J Exp Med.
.
Abstract
Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.
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