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. 1950 Jan 1;91(1):1-13.
doi: 10.1084/jem.91.1.1.

Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody

A H COONS, M H KAPLAN

Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody

A H COONS et al. J Exp Med. .

Abstract

Improvements in a method for the specific microscopic localization of antigen in tissue cells are described. This method employs antibody labelled with fluorescein isocyanate as a histochemical stain, the specific antigen-antibody precipitate being made visible under the fluorescence microscope. Two isomeric series derived from nitrofluorescein are described.

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