Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1950 Jan 1;91(1):65-86.
doi: 10.1084/jem.91.1.65.

Lack of identity in neutralizing and hemagglutination-inhibiting antibodies against influenza viruses

D L WALKER, F L HORSFALL Jr

Lack of identity in neutralizing and hemagglutination-inhibiting antibodies against influenza viruses

D L WALKER et al. J Exp Med. .

Abstract

There is an exponential linear relationship between the quantity of influenza virus neutralized and the quantity of immune serum employed in in ovo neutralization. The slope of the neutralization line is extremely steep. The concentration of neutralizing antibody can be measured with considerable precision in ovo if the constant virus-varying serum technique is utilized. The amounts of hemagglutination-inhibiting and neutralizing antibodies which are absorbed by a given quantity of influenza virus (PR8) were found to be predictable and the degree of reactivity of these two antibodies was shown to be directly related to the extent of immunization. It was demonstrated that there are marked discrepancies in correlation between antibody titers obtained by in vitro hemagglutination-inhibition and in vivo neutralization techniques and that neutralizing antibody is preferentially absorbed by a given quantity of virus. Inasmuch as the results were found not to be attributable to peculiarities of the techniques employed, it appears that the antibodies measured by hemagglutination-inhibition in vitro and by neutralization in vivo are not identical.

PubMed Disclaimer

References

    1. J Exp Med. 1935 Mar 31;61(4):563-91 - PubMed
    1. J Exp Med. 1935 Oct 31;62(5):697-720 - PubMed
    1. J Exp Med. 1939 Jul 31;70(2):209-22 - PubMed
    1. J Exp Med. 1943 Nov 1;78(5):347-66 - PubMed
    1. J Exp Med. 1944 Jun 1;79(6):633-47 - PubMed