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. 1979 Feb 15;35(2):281-3.
doi: 10.1007/BF01920665.

A simple technique for testing the in vitro response of rabbit lymphocytes to PHA and allogeneic cells

A simple technique for testing the in vitro response of rabbit lymphocytes to PHA and allogeneic cells

P Cornu et al. Experientia. .

Abstract

Lymphocytes of rabbits can be separated from small quantities of heparinized whole blood using a simple density gradient of Ficoll-Ronpacon 1.09. This separation technique yields a pure suspension of viable cells allowing reproducible results from cultures stimulated either with PHA or allogeneic lymphocytes isolated by the same technique.

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