Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1992 Mar 16;299(3):273-7.
doi: 10.1016/0014-5793(92)80130-9.

Molecular cloning and functional expression of rat leukotriene A4 hydrolase using the polymerase chain reaction

N Makita  1 , C D Funk, E Imai, R L Hoover, K F Badr
Affiliations

Molecular cloning and functional expression of rat leukotriene A4 hydrolase using the polymerase chain reaction

N Makita et al. FEBS Lett. .

Abstract

We isolated a cDNA encoding rat leukotriene A4 (LTA4) hydrolase from mesangial cells by the polymerase chain reaction according to the human amino acid sequence. The deduced amino acid sequence shows that rat LTA4 hydrolase is a 609 amino acid protein with an Mr 69 kDa. Comparison of human LTA4 hydrolase revealed 93% homology, and include zinc-binding motifs of aminopeptidases. COS-7 cells transfected with the cDNA revealed substantial LTA4 hydrolase activity, and their activities were abolished by preincubation with captopril, representing the first reported cDNA expression of recombinant enzyme in mammalian cells. RNA blot analysis indicated that LTA4 hydrolase was expressed in glomerular endothelial, epithelial and mesangial cells.

PubMed Disclaimer

Publication types

Substances

LinkOut - more resources