General function and endocrine control of the posterior lymph hearts in Bufo marinus and Rana catesbeiana

Abstract

The effects of hypervolemia and graded increases in arginine vasotocin (AVT), angiotensin II (ANGII), and atrial natriuretic peptide (ANP) on lymph heart pressure (P(lh)) and rate (f(lh)) were examined in Bufo marinus and Rana catesbeiana. The P(lh) and f(lh) for normally hydrated B. marinus at rest were 1.45+/-0.01 kPa and 52.8+/-0.38 beats min(-1). The P(lh) and f(lh) were significantly lower in R. catesbeiana, 1.05+/-0.01 kPa and 48.4+/-0.35 beats min(-1). Hypervolemia, induced by intravenous infusion of isotonic saline, stopped the lymph hearts at volumes of 0.48%+/-0.06% and 0.32%+/-0.04% body mass in B. marinus and R. catesbeiana, respectively, equivalent to an 8% increase of their respective plasma volumes. ANP had no effect on P(lh) or f(lh) at any of the dosages tested. ANGII decreased f(lh) in both species, approximating the physiological range of concentrations. AVT, at physiological concentrations, increased P(lh) 48% in B. marinus and 38% in R. catesbeiana without changing f(lh) in either species. At higher than physiological dosages, P(lh) and f(lh) in both species declined. The results suggest that AVT, normally released during hemorrhage and dehydration, would increase lymph heart output and help compensate for the hypovolemia. This is a contrary result to previous work using supraphysiologic doses of AVT.