Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2004 Oct;78(20):10960-6.
doi: 10.1128/JVI.78.20.10960-10966.2004.

Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome

Susan M Varnum  1 , Daniel N Streblow, Matthew E Monroe, Patricia Smith, Kenneth J Auberry, Ljiljana Pasa-Tolic, Dai Wang, David G Camp 2nd, Karin Rodland, Steven Wiley, William Britt, Thomas Shenk, Richard D Smith, Jay A Nelson
Affiliations

Identification of proteins in human cytomegalovirus (HCMV) particles: the HCMV proteome

Susan M Varnum et al. J Virol. 2004 Oct.

Erratum in

  • J Virol. 2004 Dec;78(23):13395

Abstract

Human cytomegalovirus (HCMV), a member of the herpesvirus family, is a large complex enveloped virus composed of both viral and cellular gene products. While the sequence of the HCMV genome has been known for over a decade, the full set of viral and cellular proteins that compose the HCMV virion are unknown. To approach this problem we have utilized gel-free two-dimensional capillary liquid chromatography-tandem mass spectrometry (MS/MS) and Fourier transform ion cyclotron resonance MS to identify and determine the relative abundances of viral and cellular proteins in purified HCMV AD169 virions and dense bodies. Analysis of the proteins from purified HCMV virion preparations has indicated that the particle contains significantly more viral proteins than previously known. In this study, we identified 71 HCMV-encoded proteins that included 12 proteins encoded by known viral open reading frames (ORFs) previously not associated with virions and 12 proteins from novel viral ORFs. Analysis of the relative abundance of HCMV proteins indicated that the predominant virion protein was the pp65 tegument protein and that gM rather than gB was the most abundant glycoprotein. We have also identified over 70 host cellular proteins in HCMV virions, which include cellular structural proteins, enzymes, and chaperones. In addition, analysis of HCMV dense bodies indicated that these viral particles are composed of 29 viral proteins with a reduced quantity of cellular proteins in comparison to HCMV virions. This study provides the first comprehensive quantitative analysis of the viral and cellular proteins that compose infectious particles of a large complex virus.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Characterization of HCMV virion and dense body preparations. (A) Electron microscopy of negatively stained HCMV virion and dense body preparations. Magnification, ×8,400. (B) Analysis of the proteins that constitute the purified HCMV particle preparations. Proteins were separated using NuPAGE MOPS gradient gels and visualized by Coomassie blue staining.
FIG. 2.
FIG. 2.
StoS ORF map. The genome of HCMV is represented by the line. Newly identified StoS ORFs are indicated by solid arrows, and ORFs that were recognized previously in AD169 are marked by open arrows.
FIG. 3.
FIG. 3.
Abundant peptides identified as UL80-specific peptides by LC-MS/MS and/or by FTICR associated with virions.
See this image and copyright information in PMC

References

    1. Adair, R., E. R. Douglas, J. B. Maclean, S. Y. Graham, J. D. Aitken, F. E. Jamieson, and D. J. Dargan. 2002. The products of human cytomegalovirus genes UL23, UL24, UL43 and US22 are tegument components. J. Gen. Virol. 83:1315-1324. - PubMed
    1. Adkins, J. N., S. M. Varnum, K. J. Auberry, R. J. Moore, N. H. Angell, R. D. Smith, D. L. Springer, and J. G. Pounds. 2002. Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry. Mol. Cell Proteomics 1:947-955. - PubMed
    1. Baldick, C. J., Jr., and T. Shenk. 1996. Proteins associated with purified human cytomegalovirus particles. J. Virol. 70:6097-6105. - PMC - PubMed
    1. Belov, M. E., G. A. Anderson, M. A. Wingerd, H. R. Udseth, D. C. Tang, K. R. Prior, M. A. Swanson, E. F. Buschbach, E. Stritmatter, R. J. Moore, and R. D. Smith. 2004. An automated high performance capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometer for high-throughput proteomics. J. Am. Soc. Mass Spectrom. 15:212-232. - PubMed
    1. Benko, D. M., R. S. Haltiwanger, G. W. Hart, and W. Gibson. 1988. Virion basic phosphoprotein from human cytomegalovirus contains O-linked N-acetylglucosamine. Proc. Natl. Acad. Sci. USA 85:2573-2577. - PMC - PubMed

Publication types

LinkOut - more resources