Localization and connectivity of the lateral amygdala in anuran amphibians

Abstract

On the basis of chemoarchitecture and gene expression patterns in the amphibian amygdaloid complex, new subdivisions have been proposed and compared with their counterparts in amniotes. Thus, a portion of the ventral pallium of anurans has been tentatively named "lateral amygdala" (LA) and compared with the basolateral complex of mammals. To strengthen the putative homology, we have analyzed the pattern of afferent and efferent connections of the LA in the anurans Rana perezi and Xenopus laevis. Tract-tracing techniques with dextran amines were used under in vivo and in vitro conditions. The results showed important connections with the main olfactory bulb, via the lateral olfactory tract. In addition, abundant intratelencephalic connections, via the rostral branch of the stria terminalis, were revealed, involving mainly the basal ganglia, septal nuclei, bed nucleus of the stria terminalis, and especially other amygdaloid nuclei. Nontelencephalic connections were found from the dorsal thalamus and parabrachial area and, in particular, from the hypothalamus through the caudal branch of the stria terminalis. All these results strongly suggest that the LA in anurans is a multimodal area in the ventral pallium that shares many hodological features with the amygdaloid ventropallial derivatives of the basolateral complex of amniotes.